Optimization in sample stacking for the measurement of short chain organic acids in serum of natural rubber latex by capillary electrophoresis |
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Authors: | V Galli |
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Affiliation: | Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, 13071 Ciudad Real, Spain |
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Abstract: | It is of interest to measure the short chain organic acid content in natural latex serum, and capillary electrophoresis (CE) has proved to be a good tool for this study. However, for some acids, a higher sensitivity than that provided by the standard methods was needed. Therefore, a stacking effect was optimised and applied both for standards and real samples. Large-volume injection of sample solutions prepared in low conductivity matrices containing 50% acetonitrile (v/v) and 0.5% NaCl (w/v) gave the best results with enhancement factors over 17 and recoveries ranging from 83±14 to 122±4%. |
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Keywords: | AcN, acetonitrile AcN-NaCl, acetonitrile-NaCl mixture BGE, background electrolyte Cinj, injected solute concentration CTAB, cetyltrimethylammonium bromide EOF, electroosmotic flow FASS, field-amplified sample stacking IC, ionic chromatography ITP, isotacophoresis KRF, Kohlrausch regulation function LOQ, limit of quantification LVSS, large-volume sample stacking NSM, normal stacking mode Sc, corrected peak area Spl, sample Std, standard |
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