Structure elucidation of uniformly 13C labeled small molecule natural products |
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| Authors: | Mikhail Reibarkh Thomas P. Wyche Josep Saurí Tim S. Bugni Gary E. Martin R. Thomas Williamson |
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| Affiliation: | 1. Process and Analytical Chemistry, NMR Structure Elucidation Group, Merck Research Laboratories, Rahway, NJ, United States;2. School of Pharmacy, University of Wisconsin—Madison, Madison, WI, United States |
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| Abstract: | Utilization of 2H, 13C, and 15N isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The widespread availability of inexpensive, uniformly 13C enriched glucose now makes it possible to isolate uniformly 13C labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly 13C labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | U‐13C labeled CT‐HSQC 13C– 13C COSY and COSYLR natural products structure elucidation enterocin |
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