大鼠脑组织含磷代谢物随年龄变化的NMR研究

随着人口老龄化的不断加剧,衰老相关疾病已成为全球关注的问题.神经系统功能的退行性改变居于衰老相关疾病的首位,其机制尚不明了.该文旨在通过核磁共振磷谱(~(31)P NMR)检测不同月龄大鼠脑组织中小分子物质的改变,明确能量相关的含磷化合物随大鼠年龄增长的变化,从而揭示脑组织中年龄相关代谢物的变化规律.该研究萃取了不同年龄(幼年、中年及老年)Sprague-Dawley(SD)大鼠脑组织中的小分子物质,进行了~(31)P NMR检测分析.结果显示:老年大鼠脑组织中能量代谢相关物质——磷酸肌酸的含量显著升高,而磷脂酰胆碱及磷酸肌醇含量显著降低,并且出现了磷酸化葡萄糖.这一发现有助于理解衰老相关的脑结构和功能下调,为神经退行性疾病的发生提供依据.

NMR-Based Analysis of Age-Related Changes of Phosphate Metabolites in Rat Brain

With advances in population aging, age-related diseases have become a global problem. Neurodegenerative disease is one type of leading aging associated diseases, the mechanism of which remains elusive. In the present study,31P NMR spectroscopy was used to characterize alterations of small molecules in rat brain for different age group, and identify alterations of energy associated phosphorylated compounds with aging, which will be helpful for interpreting age-related metabolism in brain. Results showed that energy related metabolite phosphocreatine increased significantly, whereas phosphatidylcholine and phosphorylcholine reduced significantly in aged rat brain. Interestingly, glucose phosphates appeared in aged rat brain. This study demonstrated the alterations of phospholipid metabolism in rat brain with aging, which may help to understand the development of neurodegeneration, and these metabolites may be used as molecular markers for monitoring neurodegenerative disorders.