Development of an accelerated low-pH reversed-phase liquid chromatography column stability test |
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Authors: | Trammell Brian C Boissel Cheryl A Carignan Christina O'Shea Daniel J Hudalla Christopher J Neue Uwe D Iraneta Pamela C |
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Institution: | Waters Corporation, 34 Maple Street, Milford, MA 01757, USA. |
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Abstract: | The important experimental design criteria for an accelerated low-pH RPLC column stability test are discussed. The influence of method variables on the amount and rate of retention-loss and the final optimized parameters for the accelerated low-pH RPLC stability test are presented. The retention-loss curves for selected C8 and C18 stationary phases are compared. These studies indicate that ligand chain length, functionality and bonding density play an important role in determining the low-pH stability of a stationary phase. Additionally, elemental analysis data are used to infer the mechanism responsible for the observed retention-loss under low-pH conditions. |
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