| 东北虎Sox3和Sox4基因的保守区序列分析 |
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| 引用本文： | 杜启艳,常重杰,牛红星.东北虎Sox3和Sox4基因的保守区序列分析[J].河南师范大学学报(自然科学版),2003,31(1):78-81. |
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| 作者姓名： | 杜启艳 常重杰 牛红星 |
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| 作者单位： | 河南师范大学生命科学学院,河南,新乡,453002 |
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| 基金项目： | 河南省科技厅自然科学基金资助项目 (编号 :9940 10 80 0 ) |
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| 摘 要： | 应用特异于HMG－box区域的兼并引物，以基因组DNA为模板扩增了东北虎的SOX基因，在扩增产物中发现一条220bp的扩增带。经过克隆、序列测定和同源性检索，发现一个基因片段与人、小鼠、鸡和爪蟾Sox3的同源性分别为95％、95％、92％和88％；与人和小鼠SRY基因的同源性分别为75％和73％。因此该片段应当为东北虎的Sox3基因片段，被命名为PtSox3。另一片段与人、小鼠、爪蟾和大鼠Sox4的同源性分别为98％、97％、95％和94％；与人和小鼠SRY基因的同源性分别为56％和54％被命名为PtSox4。
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| 关 键 词： | 保守区 序列分析 东北虎 Sox3基因 Sox4基因 同源性 发育基因 |
| 文章编号： | 1000-2367(2003)01-0078-04 |
| 修稿时间： | 2001年12月15 |
The Sequence Analysis of Sox3 and Sox4 HMG-box Region in PANTHERA TIGRIS ALTAICA |
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| DU Qi-yan,CHANG Zhong-jie,NIU Hong-xing.The Sequence Analysis of Sox3 and Sox4 HMG-box Region in PANTHERA TIGRIS ALTAICA[J].Journal of Henan Normal University(Natural Science),2003,31(1):78-81. |
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| Authors: | DU Qi-yan CHANG Zhong-jie NIU Hong-xing |
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| Abstract: | The SOX genes of Panthera tigris altaica Temminck were detected by a pair of degenerate primers. The degenerate primers were special to the SOX genes family within the HMG.box. With the genomic DNA as template, one specific band was observed with the length of 220bp. One fragment of DNA was founded with the identities to HSOX3, Msox3, Csox3 and XlSox3 95%,95%,92% and 88% respectively. The similarities of this fragment to HSRY and Msry were 75% and 73% respectively. Therefore, it was part of SOX3 in Panthera tigris altaica Temminck, and was named as PtSox3. The identities of another fragment to SOX4 of human, mouse, Xenopus laevis and rat were 98%,97%,95% and 94% respectively. The similarities of this fragment to HSRY and Msry were 56% and 54% respectively. So, it was named PtSox4. |
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| Keywords: | Panthera tigris altaica SOX3 SOX4 |
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