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Complement activation by sulfonated poly(ethylene glycol)-acrylate copolymers through alternative pathway |
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| Authors: | Jang Hong Seok Ryu Kyu Eun Ahn Woong Shick Chun Heung Jae Dal Park Hyung Park Ki Dong Kim Young Ha |
| Affiliation: | aDepartment of Therapeutic Radiology, Uijeongbu St. Mary's Hospital, College of Medicine, Catholic University, Uijeongbu 480-130, Korea bDepartment of Biomedical Sciences, College of Medicine, Catholic University, Seoul 137-701, Korea cDepartment of Obstetrics and Gynecology, Kangnam St. Mary's Hospital, College of Medicine, Catholic University, Seoul 137-701, Korea dInstitute of Cell and Tissue Engineering, College of Medicine, Catholic University, Seoul 137-701, Korea eChemistry and Biotechnology Exam. Bureau, Korean Intellectual Property Office (KIPO), Government Complex-Daejeon, Daejeon 302-701, Korea fDepartment of Molecular Science and Technology, University of Ajou, Suwon 442-749, Korea gDepartment of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Korea |
| Abstract: | Previously, novel poly(ethylene glycol) (PEG) and sulfonated PEG acrylate (PEG-SO3A/OA) copolymers were prepared as coating and/or blending materials for biomedical applications. Surfaces modified with copolymers exhibited increased anti-coagulation properties and decreased plasma adsorption level due to increased hydrophilic properties and reorientation characteristics of PEG/PEG-SO3A chains in water phase. As continuation study, anti-complement effects of PEG-SO3/OA copolymers were investigated in vitro, and compared with those of low-density polyethylene (LDPE) and PEG/OA. C3 activation by PEG-SO3/OA samples was lower than that by PEG/OA samples, which was attributed to decreased surface nucleophile level of samples. PEG-SO3/OA samples increased inhibition of Bb production, resulting in decreased C5 activation. Owing to reduced activations of C3 and C5, PEG-SO3/OA samples markedly decreased SC5b-9 levels in plasma. |
| Keywords: | Biomaterials Poly(ethylene glycol) Acrylate Copolymerization Sulfonation complement activation Alternative pathway |
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