Dosage polarographique du brome a l'echelle du nanogramme: Application à la determination du brome sanguin et urinairePolarographic determination of bromide at nanomolar levels. Application to the determination of bromide in blood and urine |
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Authors: | JJ Vallon Y Pegon M Accominotti |
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Institution: | 1. Département de Chimie Analytique, Faculté de Pharmacie; Laboratoire de Biochimie, Toxicologie et Analyse des Traces, Hôpital Edouard Herriot, 69373 LyonFrance |
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Abstract: | Polarographic determination of bromide at nanomolar levels. Application to the determination of bromide in blood and urine.Colorimetric methods for bromide determination lack adequate sensitivity for normal levels in biological fluids. A sensitive amplification process is recommended: bromide is oxidized to bromate with hypochlorite; after reaction between bromate and excess of bromide, the bromine formed is extracted into chloroform and then reduced to bromide by ammonia; these different steps can be repeated. Alternating current polarography of bromate allows selective evaluation in biological fluids. The detection limit is 10-6 M and can be reduced to 10-9 M with further amplification steps. The effects of iodide and of instrumental parameters are discussed. |
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