| 用MALDI-TOF-MS法快速测定天花粉蛋白分子量 |
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| 引用本文： | 季怡萍,刘淑莹.用MALDI-TOF-MS法快速测定天花粉蛋白分子量[J].分析测试技术与仪器,1999,5(2):94-95. |
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| 作者姓名： | 季怡萍 刘淑莹 |
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| 作者单位： | 中国科学院长春应用化学研究所 |
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| 摘 要： | 采用ＭＡＬＤＩ－ＴＯＦ－ＭＳ法快速测定了天花粉蛋白的分子量，并讨论和对比了三种不同基质对其影响，认为用基质芥子酸是最佳适宜条件。实验结果表明本方法优于其它传统的测定生物大分子分子量方法。
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| 关 键 词： | 测定 分子量 MALDI TOF MS 天花粉蛋白 TCS |
| 收稿时间： | 1998/12/19 0:00:00 |
| 修稿时间： | 1999/2/20 0:00:00 |
Rapid Molecular Weight Determination of Trichosanthin by MALDITOFMS |
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| JI Yiping and LIU Shuying.Rapid Molecular Weight Determination of Trichosanthin by MALDITOFMS[J].Analysis and Testing Technology and Instruments,1999,5(2):94-95. |
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| Authors: | JI Yiping and LIU Shuying |
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| Institution: | Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022;Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022 |
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| Abstract: | The molecular weight of trichosanthin was determinated rapidly by MALDITOFMS. The effects of three kinds of matrices were compared and diccussed, sinapinic acid was approved to be the best matrix for trichosanthia. The experiment results showed that MALDITOFMS me thod was superior to the traditional method of molecular weight determination of biological macro molecules. |
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| Keywords: | matrixassisted laser desorption ionization timeof flight mass spectrometry trichosanthin |
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