Synthesis,characterization, X-ray diffraction,antimicrobial and in vitro cytotoxicity studies of 7a-Aza-B-homostigmast-5-eno [7a,7-d] tetrazole |
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Authors: | Mahboob Alam Shahab A.A. Nami Ahmad Husain Dong-Ung Lee Soonheum Park |
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Affiliation: | 1. Division of Bioscience, Dongguk University, Gyeongju 780-714, Republic of Korea;2. Department of Chemistry, Dongguk University, Gyeongju 780-714, Republic of Korea;3. Department of Kulliyat, Faculty of Unani Medicine, Aligarh Muslim University, Aligarh 202002, India;4. Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India |
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Abstract: | The synthesis, spectral characterization, crystal structure and antimicrobial activity of the novel synthetic molecule 7a-Aza-B-homostigmast-5-eno [7a,7-d] tetrazole, C29H48N4 has been reported. The structure has also been determined by X-ray diffraction technique using direct method and was refined on F2 by the full-matrix least-squares. Crystals are orthorhombic and their space group is P212121, with a = 7.230(3), b = 31.451(13), c = 11.974(5) (Å), α = β = γ = 90°. It can be conveniently obtained by the reaction of 7-Oxostigmast-5-ene with hydrazoic acid. The molecule has also been screened for its possible in vitro antimicrobial activity against Staphylococcus aureus, Streptococcus mutans, Streptococcus pyogenes, Staphylococcus epidermidis, Bacillus cereus, Corynebacterium xerosis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa (MTCC 424). Minimum inhibitory concentration (MIC) of the synthesized compound has also been evaluated. The highest activity is observed against C. xerosi and P. vulgaris. Moreover, the compound has also been screened for its in vitro cytotoxicity against human colon carcinoma cell line, HCT116 and human liver hepatocellular carcinoma cell line, HepG2, using doxorubicin as standard. On the basis of its IC50 values, 7a-Aza-B-homostigmast-5-eno [7a,7-d] tetrazole was found to inhibit the cancer cells effectively. |
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