Bioorthogonal chemical tagging of protein cholesterylation in living cells |
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| Authors: | Heal William P Jovanovic Biljana Bessin Sara Wright Megan H Magee Anthony I Tate Edward W |
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| Affiliation: | Department of Chemistry, Imperial College London, Exhibition Rd., London SW7 2AZ, UK. |
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| Abstract: | We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins. |
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