| 庆大霉素与水溶性CdTe量子点的荧光共振能量转移作用 |
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| 引用本文： | 李金贵,朱奎,徐飞,江海洋,丁双阳. 庆大霉素与水溶性CdTe量子点的荧光共振能量转移作用[J]. 光谱学与光谱分析, 2009, 29(11): 3070-3074. DOI: 10.3964/j.issn.1000-0593(2009)11-3070-05 |
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| 作者姓名： | 李金贵 朱奎 徐飞 江海洋 丁双阳 |
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| 作者单位： | 1. 扬州大学兽医学院,江苏,扬州,225009
2. 中国农业大学动物医学院/国家兽药残留基准实验室,北京,100193 |
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| 基金项目： | 国家自然科学基金项目，国家"十一五"科技支撑计划项日 |
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| 摘 要： | 以巯基丙酸(mercaptopropionic acid,MPA)为稳定剂合成水溶性CdTe最子点(quantum dots,QDs),以CdTe QDs作为能量供体.庆大霉素(Gentamycin,GT)作为能垦受体,建立了荧光共振能量转移(fluorescence resonance energy transfer,FRET)体系.在690 nm处可见发射峰,半峰宽约10 nm,在一定范围内荧光强度与GT的含量旱线性关系,线性范围为2～20 mg·L-1,相关系数r=0.986 7.优化了不同的激发波长、pH、离子强度、时间和温度等凼素对反应的影响,并应用傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)和高效液相色谱(high-performance liquid chromatography,HPLC)分别表征了化学结构和相对专一性.结果表明巯基丙酸的巯基中S原子和羧基中氧原子与纳米微粒表面的富Cd离子发生了配位作用,CdTe QDs与GT的耦合主要是通过量子点周围巯基丙酸羧基(-COOH)中的氧原子与GT的胺基(-NH2)形成分子问氧键实现的;GT与CdTe QDs的结合率为0.35:1.研究表明GT可以作为检测CdTe QDs标记牛血清白蛋白(bovine serum albumin,BSA)的荧光增敏剂,荧光强度值增强6倍,应用前景广阔.
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| 关 键 词： | 庆大霉素 水溶性CdTe量子点 荧光共振能最转移 牛血清白蛋白 |
| 收稿时间： | 2008-10-28 |
Fluorescence Resonance Energy Transfer between Gentamycin and Water-Soluble CdTe QDs |
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| LI Jin-gui,ZHU Kui,XU Fei,JIANG Hai-yang,DING Shuang-yang . College of Veterinary Medicine of Yangzhou University,Yangzhou ,China. College of Veterinary Medicine,China Agricultural University/National Reference Laboratory for Veterinary Drug Residue,Beijing ,China. Fluorescence Resonance Energy Transfer between Gentamycin and Water-Soluble CdTe QDs[J]. Spectroscopy and Spectral Analysis, 2009, 29(11): 3070-3074. DOI: 10.3964/j.issn.1000-0593(2009)11-3070-05 |
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| Authors: | LI Jin-gui ZHU Kui XU Fei JIANG Hai-yang DING Shuang-yang . College of Veterinary Medicine of Yangzhou University Yangzhou China. College of Veterinary Medicine China Agricultural University/National Reference Laboratory for Veterinary Drug Residue Beijing China |
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| Affiliation: | LI Jin-gui1,ZHU Kui2,XU Fei2,JIANG Hai-yang2,DING Shuang-yang2 1. College of Veterinary Medicine of Yangzhou University,Yangzhou 225009,China2. College of Veterinary Medicine,China Agricultural University/National Reference Laboratory for Veterinary Drug Residue,Beijing 100193,China |
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| Abstract: | The water-soluble CdTe quantum dots (QDs) were prepared by using mercaptopropionic acid (MPA) as stabilizer in the aqueous system. Fluorescence resonance energy transfer (FRET) system was constructed between gentamycin (acceptor) and water-soluble CdTe QDs (donor). The maximal emission wavelength was 690 nm,and the line width of the fluorescence spectrum was very narrow (with the full width at half-maximum about 10 nm) and symmetric. The transfer of resonance energy from the CdTe QDs to gentamycin (GT) resu... |
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| Keywords: | Gentamycin (GT) Water-soluble CdTe quantum dots (QDs) Fluorescence resonance energy transfer (FRET) Bovine serum albumin (BSA) |
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