Application of analytical and preparative high-speed counter-current chromatography for separation of lycopene from crude extract of tomato paste |
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| Authors: | Wei Y Zhang T Xu G Ito Y |
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| Institution: | Beijing Research Center for Separation and Purification Technologies of Natural Prodiucts, Beijing Institute of New Technology Application, PR China. |
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| Abstract: | Lycopene was isolated from 100 mg of crude extract of tomato paste containing about 9% of lycopene. Analytical high-speed counter-current chromatography (HSCCC) was first used for the systematic selection of the two-phase solvent system. Then preparative HSCCC separation was performed with a nonaqueous solvent system composed of n-hexane-dichloromethane-acetonitrile at an optimum volume ratio of 10:3.5:6.5. This yielded 8.6 mg of lycopene at over 98.5% purity as determined by HPLC analysis. |
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