| 改进的三羟基吲哚法测定肾上腺素 |
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| 引用本文： | 童裳伦,吕国才,许志良. 改进的三羟基吲哚法测定肾上腺素[J]. 浙江大学学报(理学版), 2000, 27(5): 530-533 |
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| 作者姓名： | 童裳伦 吕国才 许志良 |
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| 作者单位： | [1]浙江大学环境科学系 [2]浙江大学附一医院 |
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| 基金项目： | 浙江省自然科学基金!(3990 88)，浙江省分析测试基金 |
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| 摘 要： | 本文利用改进的三羟基吲哚法测定肾上腺素. 实验表明,在pH=8.7的硼砂缓冲溶液中,肾上腺素用K3Fe(CN)6氧化后,加入巯基乙醇溶液,能稳定吲哚衍生物,从而可以在碱性条件下测定肾上腺素,同时在该条件下多巴、多巴胺不能形成发荧光的产物,不干扰它的测定,由此提出了一种灵敏、选择性测定肾上腺素的分析方法.
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| 关 键 词： | 三羟基吲哚法 肾上腺素 荧光光度法 |
| 修稿时间： | 1999-03-26 |
Determination of epinephrine by an improved modification of the trihydroxyindole method. |
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| TONG Chang|lun ,LU Guo|cai ,XU Zhi|liang. Determination of epinephrine by an improved modification of the trihydroxyindole method.[J]. Journal of Zhejiang University(Sciences Edition), 2000, 27(5): 530-533 |
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| Authors: | TONG Chang|lun LU Guo|cai XU Zhi|liang |
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| Affiliation: | 1. Department of Environmental Science, Zhejiang University, Hangzhou 310028, China; 2. The First Hospital of Zhejiang University, Hangzhou 310003, China; 3. Traditional Medical Hospital of Zhejiang, Hangzhou 310006, China |
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| Abstract: | An improved modification of the trihydroxyindole method was used to determine epinephrine in this paper. Experiments indicate that the fluorescent lutines can be stablized by the mercaptoethanol in the borax buffer(pH=8.7). So it is possible to determine epinephrine in the alkaline medium. Under the same conditions, dopa, dopamine can not form fluorescent products, therefore, they didn't interfere with the estimation of epinephrine. From this, a simple and selective method for the determination of epinephrine was put forward. |
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| Keywords: | the trihydroxyindole method epinephrine spectrofluorimetry |
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