罗丹明-四苯硼钠体系反荧光猝灭法测定盐酸西替利嗪

四苯硼钠对罗丹明B具有荧光猝灭作用,使荧光信号强度减弱甚至消失,而盐酸西替利嗪对罗丹明B-四苯硼钠体系具有反猝灭作用,又能使该体系荧光信号增强,且荧光信号增强程度与药物浓度成线性关系.该文以491 nm为激发波长,以610 nm作发射波长,测量空白液与试液荧光强度之差△F=F1-F0,根据△F值与溶液中的盐酸西替利嗪浓...

Determination of cetirizine dihydrochloride by anti-fluorescence quenching on rhodamine B-sodium tetraphenylborate system

The sodium tetraphenylboron has the fluorescence quenching effect on rhodamine B, making the fluorescence signal intensity weaken or even disappear. But cetirizine dihydrochloride has the anti-quenching effect on rhodamine B--the fluorescence signal is enhanced, and there was a linear relationship between the fluorescence signal enhancement degree and drug concentration. In the present paper, 491 and 610 nm were chosen as the excitation and emission wavelength for measurement of the fluorescence intensity difference deltaF = F1 - F0 of blank solution and test solution, and according to positive correlation between the value of deltaF and cetirizine hydrochloride concentration of test solution, a new method of anti-fluorescence quenching for the determination of cetirizine hydrochloride was established. The linear regression equation was deltaF = 0.727 5m - 2.357, correlation coefficient was 0.997 2, linear range was 3.50-129.3 mg x L(-1), the detection limit was 1.05 mg x L(-1) and RSD was 1.2%. The cetirizine hydrochloride tablets and cetirizine hydrochloride capsules from different manufacturers were determined by this method, the measured values were basically in line with the labeled amount of drugs, and the recovery rate was between 92% and 106%.