A nickel oxide amperometric detector in the chromatographic separation of amino acids |
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| Authors: | JB Kafil CO Huber |
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| Institution: | Department of Chemistry, University of Wisconsin-Milwaukke, Milwaukee, WI 53201 U.S.A. |
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| Abstract: | Reverse-phase ion-pair chromatography with a nickel oxide amperometric detector is evaluated for separation and detection of several amino acids. A simple T-junction allows post-column mixing of detector electrolyte. Mixer and detector dimensions are shown to contribute negligibly to band spreading. Picomole amounts of some amino acids can be detected. The system is evaluated for determination of amino acids in hydrolyzed bovine A-chain insulin and urine samples. |
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