A fluoroimmunoassay for screeing of hepatitis B surface antigen |
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| Authors: | Guido de Schrijver Roger Jozef Wieme Luc Pittoors |
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| Institution: | Laboratory of Internal Medicine, Academic Hospital, De Pintelaan 185, B-9000 Gent Belgium |
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| Abstract: | A fast, sensitive sandwich fluorimmunoassay for hepatitis B surface antigen (HBs-ag) is described. Rabbit antibodies to HBs-ag are covalently bound to the centre of a glass-fiber filter disk. The second antibody is a swine anti-human HBs-ag conjugated with β-d-galactosidase from E. coli. The substrate is 4-methylumbelliferyl-β-d-galactopyrano-side. The technique is applied with the Stratus system which does the entire test in 8 min. the detection limit is 1 ng ml?1, and the results correlate well with a radioimmunoassay technique. |
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