Characterization of micellar mobile phases for reversed-phase chromatography |
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Authors: | John S Landy John G Dorsey |
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Institution: | Department of Chemistry, University of Florida, Gainesville, FL 32611 U.S.A. |
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Abstract: | Cationic, anionic, and nonionic surfactants are characterized for their usefulness as micellar mobile phases in reversed-phase chromatography. Conditions found previously to provide optimum chromatographic efficiency for sodium dodecyl sulfate also provide high efficiency for the cationic and nonionic surfactants studied. The use of 3% n-propanol in the micellar mobile phase and column temperatures of 40°C appear to offer a broadly applicable solution to the low efficiency previously reported for micellar mobile phases. A chromatographic method for the determination of critical micelle concentrations is reported; it compares favorably with literature methods. Micellar mobile phases are shown to mimic ion-pairing mobile phases, allowing the separation of neutral solutes as well as solutes charged oppositely to the surfactant and offer a more rugged method of analysis than hydro-organic ion-pairing methods. |
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