Flow injection analysis for traces of zinc with immobilized carbonic anhydrase |
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| Authors: | Kunio Kashiwabara Toshiyuki Hobo Eigo Kobayashi Shigetaka Suzuki |
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| Institution: | Department of Industrial Chemistry, Faculty of Technology, Tokyo Metropolitan University, 2-1-1 Fukasawa, Setagaya-ku, Tokyo 158 Japan |
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| Abstract: | A small column packed with immobilized bovine carbonic anhydrase is used for determination of traces of zinc in aqueous solution, based on the measurement of recovered esterase activity of the metal-free apoenzyme after taking up zinc from the sample solution. Conditions for the removal of zinc from the immobilized enzyme and for activity measurement are established. A linear calibration graph is obtained between 1 × 10?8 and 4 × 10?7 g of zinc. Then the method is successfully applied to the determination of zinc in tea, sediment and spring water. |
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