Determination of cholesterol by flow injection analysis with immobilized cholesterol oxidase |
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Authors: | M. Masoom Alan Townshend |
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Affiliation: | Department of Chemistry, University of Hull, Hull HU6 7RX Great Britain |
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Abstract: | The injected sample passes through a column of enzyme immobilized on controlled pore glass, at pH 7.0, and the hydrogen peroxide produced is detected amperometrically. As little as 0.2 μg of cholesterol can be detected. The method is applied to blood serum, wax-wool alcohol and an extract of butter. |
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