Fluorimetric assay of redox activity in cells |
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| Authors: | Erhard Severin Joachim Stellmach Hans-Martin Nachtigal |
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| Institution: | Institut für Strahlenbiologie der Universität Münster, Hittorfstrasse 17, D-4400 Münster Federal Republic of Germany |
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| Abstract: | A simple, sensitive procedure is described for measurement of the redox activity of single cells based on cyanotolyltetrazolium chloride which is reduced to a fluorescent monoformazan. Its efficiency is studied by incubating vital ascites tumor cells or cells treated with different fixatives, under different oxygen concentrations. Redox activity (fluorescence of the formazan) and DNA content (fluorescence of Hoechst dye 333378) of ascites tumor cells are measured simultaneously for the first time, by flow cytometry. |
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