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A solid-phase chemiluminescence immunoassay for 17α-methyltestosterone |
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| Authors: | E.H.J.M. Jansen C.A. Laan R.H. van den Berg R.W. Stephany G. Zomer |
| Affiliation: | Laboratory for Endocrinology, National Institute for Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven The Netherlands;Laboratory for Clinical Chemistry and Haematology, National Institute for Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven The Netherlands |
| Abstract: | A solid-phase immunoassay based on chemiluminescence detection is described for the anabolic steroid 17α-methyltestosterone (MT). The reproducibility is increased substantially by introduction of the solid-phase principle and the assay can therefore be used to quantify MT in bovine urine. The detection limit is 10 pg. |
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