Simultaneous determination of l(+)- and d(−)-lactic acid by use of immobilized enzymes in a flow-injection system |
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Authors: | Toshio Yao Tamotsu Wasa |
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Affiliation: | Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Mozu-Umemachi, Sakai 591 Japan |
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Abstract: | The system comprises l(+)- and d(?)-lactate dehydrogenase reactors in parallel and a diaphorase electrode. Separate peaks are obtained for l(+)- and d(?)-lactic acid. The peak current is linearly related to the concentration of both isomers in the range 1 × 10?5?2 × 10?3 M. |
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