| 交联结构对肝素/壳聚糖层层组装多层膜内皮细胞相容性的影响 |
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| 引用本文： | 侯悦,林全愧,计剑,沈家骢.交联结构对肝素/壳聚糖层层组装多层膜内皮细胞相容性的影响[J].高等学校化学学报,2008,29(9):1890-1894. |
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| 作者姓名： | 侯悦 林全愧 计剑 沈家骢 |
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| 作者单位： | 浙江大学高分子科学与工程学系,教育部高分子合成与功能构造重点实验室,杭州,310027 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973计划)，浙江省科技计划 |
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| 摘 要： | 采用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺交联技术对具有抗凝血抗菌作用的肝素/壳聚糖多层膜进行交联, 研究了交联结构对多层膜稳定性和血管内皮细胞亲和性的影响. QCM-D结果显示, 交联可有效地提高多层膜的稳定性, 在模拟人体血液流速(3.0 cm/s)下保持良好的稳定. 体外内皮细胞的研究结果显示, 多层膜的交联可有效地调节肝素/壳聚糖多层膜表面粘弹性, 并显著增加内皮细胞的粘附与生长. 交联的肝素/壳聚糖多层膜有望成为理想的心血管功能界面涂层材料.
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| 关 键 词： | 肝素 壳聚糖 内皮细胞 交联 层层自组装 |
| 收稿时间： | 2008-03-10 |
Improvement of Endothelialization of(Heparin/Chitosan) Multilayer Films via Chemical Cross-Linking |
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| HOU Yue,LIN Quan-Kui,JI Jian,SHEN Jia-Cong.Improvement of Endothelialization of(Heparin/Chitosan) Multilayer Films via Chemical Cross-Linking[J].Chemical Research In Chinese Universities,2008,29(9):1890-1894. |
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| Authors: | HOU Yue LIN Quan-Kui JI Jian SHEN Jia-Cong |
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| Institution: | Key Laboratory of Macromolecule Synthesis and Functionalzation of Minster of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | Heparin(HEP) /chitosan(CHI) multilyayer films were cross-linked by 1-ethyl-3-(3-dimethy-laminopropyl) carbodiimide(EDC) technique.The stability and endothelial cell activities of multilayer films were investigated.QCM-D test indicates that the cross-linking could improve the stablility of the multilayer films.The multilayer films had good stability when simulating human blood flow rate-3.0 cm/s.In vitro endothelial cell tests suggest that cross-linking could effectively modify the viscoelasticity of(HEP/CHI) multilayer film surface and improve the adhesion and proliferation behavior of endothelial cells.The cross-linked(HEP/CHI) multilayered film might have a great potential as a coating for cardiovascular materials. |
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| Keywords: | Heparin Chitosan Endothelial cell Cross-linking Layer-by-layer self-assembly |
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