Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer |
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| Authors: | I Y Goryacheva E Y Basova C Van Peteghem S A Eremin L Pussemier J-C Motte S De Saeger |
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| Institution: | (1) Department of Common and Inorganic Chemistry, Chemistry Faculty, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia;(2) Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium;(3) Department of Chemical Enzymology, Chemistry Faculty, Moscow State University, Leninskie Hills, 1, 119992 Moscow, Russia;(4) Veterinary and Agrochemical Research Centre (CODA-CERVA), Leuvensesteenweg 17, 3080 Tervuren, Belgium |
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| Abstract: | A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical
method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection
in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 μg
L-1. Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened
with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid
and cost-effective screening tool, thus contributing to better health protection of consumers.
Figure Gel-based immunoassay of spiked beer samples. |
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| Keywords: | Immunoassay Screening Mycotoxin Food control Visual detection Beer Ochratoxin A |
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