| 利用响应面分析法优化葛根异黄酮提取工艺的研究 |
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| 引用本文： | 张宏川,刘思洋,王蕾,顾健,谭睿.利用响应面分析法优化葛根异黄酮提取工艺的研究[J].西南民族大学学报(自然科学版),2015,41(6):695-700. |
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| 作者姓名： | 张宏川 刘思洋 王蕾 顾健 谭睿 |
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| 作者单位： | 西南民族大学民族医药研究院,西南民族大学民族医药研究院,西南民族大学民族医药研究院,西南民族大学民族医药研究院,西南交通大学生命科学与工程学院 |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2013ZX09103-002-014);西南民族大学研究生学位点建设项目(2015XWD-S0703);西南民族大学研究生“创新型科研项目”(CX2015SZ036)。 |
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| 摘 要： | 本文通过响应面分析方法确定葛根异黄酮最佳提取条件.以葛根异黄酮提取率为指标,利用响应面分析法分别以超声时间、乙醇浓度、料液比及提取时间等4因素为影响因子,考察各影响因子对葛根异黄酮提取率的影响.结果表明优化提取条件后葛根异黄酮的最佳提取工艺条件为:乙醇浓度为91.16%,超声时间16.59 min,料液比15.42:1,提取时间1.35 h.理论提取率为1.125 mg/g,实际测得提取率为1.025 mg/g.Box-Behnken实验设计方法可以较好地对葛根异黄酮提取工艺进行优化.
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| 关 键 词： | 葛根 葛根异黄酮 响应面分析法 |
| 收稿时间： | 2015/10/6 0:00:00 |
| 修稿时间： | 2015/10/20 0:00:00 |
Isoflavones extraction technology optimized by response surface methodology |
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| ZHANG Hong-chuan,LIU Si-yang,Wang Lei,GU Jian and Tan Rui.Isoflavones extraction technology optimized by response surface methodology[J].Journal of Southwest University for Nationalities(Natural Science Edition),2015,41(6):695-700. |
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| Authors: | ZHANG Hong-chuan LIU Si-yang Wang Lei GU Jian and Tan Rui |
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| Institution: | Institute of National Medicine,Southwest University for Nationalities,Institute of National Medicine,Southwest University for Nationalities,Institute of National Medicine,Southwest University for Nationalities,Institute of National Medicine,Southwest University for Nationalities,School of Life Science and Engineering, Southwest Jiaotong University |
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| Abstract: | The article identifies the optimum conditions for the extraction of isoflavones via response surface analysisi.Based on the pueraria isoflavone extraction rate index ,response surface analysis method was used to four factors including ultrasound time ,ethanol concentration,solid-liquid ratio and extraction time,and to inspect each factou on the extraction rate of isoflavones.The results showed that the optimized extraction conditions of isoflavones were as follows:ethanol condition was 91.16% , ultrasonic time 16.59 ,liquid ratio 15.42: 1, extraction time 1.35 h; Theory extraction rate of 1.125 mg / g, The actual extraction rate measured reached 1.025 mg / g. Box-Behnken experimental design method can to optimize the extraction technology of isoflavones. |
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| Keywords: | pueraria isoflavones response surface methodology |
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