Determination of amine-containing phosphonic acids and amino acids as dansyl derivatives |
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Authors: | N F Zelenkova N G Vinokurova A A Leontievskii |
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Institution: | 1.URAN Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia;2.Pushchino State University,Pushchino, Moscow oblast,Russia |
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Abstract: | Conditions are selected for the analytical separation of (N-phosphonomethyl glycine), products of its microbiological conversion, glutamic acid, and alanine as dansyl derivatives using
reversed-phase HPLC: column (250 × 4.6 mm) ReproSil-PAH EPA; mobile phase, methanol + 20 mM CH3COONa (pH 5.1) (20: 80); rate of mobile phase, 1 mL/min; working detector wavelength, 330 nm. The duration of separation is
35 min. The lower limits of the analytical range (in ng) for dansyl derivatives are as follows: glyphosate, 8.2; aminomethyl
phosphonic acid, 24.2; glutamic acid, 9.4; alanine, 12.6: glycine, 17.7; and sarcosine, 19.3. The TLC study of dansyl derivatives
of amino acids was performed on sorbfil plates PTSH-P-V using two-dimensional chromatography in the systems ethyl acetate-isopropanol-24%
aqueous ammonia (45: 35: 20) in the first direction and chloroform-methanol-acetic acid (25: 5: 1) in the second one. For
determining phosphonic acids (glyphosate and aminomethyl phosphonic acid), a version of one-dimensional chromatography with
the sequential use of two systems, chloroform-methanol-acetic acid (25: 5: 0.2) and ethanol-24% aqueous ammonia (7: 3), was
proposed. |
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