An efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure D-tert-leucine |
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| Authors: | Hummel Werner Kuzu Mutlu Geueke Birgit |
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| Institution: | Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universit?t Düsseldorf, im Forschungszentrum Jülich, 52426 Jülich, Germany. w.hummel@fz-juelich.de |
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| Abstract: | reaction: see text] d-tert-Leucine was prepared with an enantiomeric excess of >99% by an enzyme-catalyzed oxidative resolution of the racemic mixture of dl-tert-leucine with use of leucine dehydrogenase. The l-amino acid was oxidized completely due to coupling of the primary reaction with a highly efficient irreversible NAD(+)-regenerating step by NADH oxidase. |
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