硅胶整体柱芯的制备方法研究

目前，新型色谱填充材料发展很快，但传统的高效液相色谱(HPLC)颗粒型填充柱存在缺陷，难以实现既高效又快速的分离，Minakuchi等制备了连续的硅胶整体柱，该柱同时具有通孔和中孔双孔结构，孔容量大大高于填充柱，因此，该柱具有高速、低压、高效、适于溶剂及流速梯度洗脱等优点，适合高通量分析和快速分离，

Preparation Approach of Monolithic Silica Column

A new approach of preparing monolithic silica column was founded by adopting an improved sol-gel technology with tetramethoxysilane and polyethylene glycol as the matrix materials. The reason of resulting in crack and deformation of monolithic column was explained. By using N,N-dimethylformamide and a heat treatment procedure, the problems of crack and deformation in preparing the column were solved and the drying time was shortened greatly. In addition, the methods of fabricating mesopore and the effects of porogen solvent of different concentrations on monolithic silica column were investigated. The results show that the column prepared possessed bimodal pores structures with micrometer-size co-continuous through-pores and silica skeletons which have nanometer-size mesopores, straight appearance, high porosity, good chemical and mechanical stability. The characteristics would favour the separation with a rapid speed and high efficiency for both small molecules and macromolecules.