| 光谱法研究山奈酚与ct-DNA的相互作用 |
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| 引用本文： | 肖骁,张慧娟,庞思平. 光谱法研究山奈酚与ct-DNA的相互作用[J]. 光谱实验室, 2009, 26(5): 1211-1214 |
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| 作者姓名： | 肖骁 张慧娟 庞思平 |
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| 作者单位： | 北京理工大学生命学院北京中关村南大街5号,100081;北京理工大学材料学院北京中关村南大街5号,100081 |
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| 基金项目： | 庆贺于永忠教授85华诞，国家自然科学基金 |
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| 摘 要： | 利用紫外-可见吸收光谱和荧光光谱研究了黄酮类化合物山奈酚与小牛胸腺DNA（ct-DNA）之间的相互作用。随着DNA的加入,山奈酚的吸收光谱逐渐发生明显的红移和减色效应,通过双倒数法拟合分析,得到山奈酚与ct-DNA的结合常数为1.2×10^4。山奈酚自身荧光很弱,与DNA结合后其荧光明显增强,表明山奈酚可以作为荧光探针来开展与生物大分子相互作用的研究。
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| 关 键 词： | 山奈酚 小牛胸腺DNA(ct-DNA) 紫外-可见吸收光谱 荧光光谱 |
A Spectroscopic Study of Interaction between Kaempferol and ct-DNA |
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| XIAO Xiao,ZHANG Hui-Juan,PANG Si-Ping. A Spectroscopic Study of Interaction between Kaempferol and ct-DNA[J]. Chinese Journal of Spectroscopy Laboratory, 2009, 26(5): 1211-1214 |
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| Authors: | XIAO Xiao ZHANG Hui-Juan PANG Si-Ping |
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| Affiliation: | School of Life Science;Beijing Institute of Technology;Beijing 100081;P.R.China;School of Material Science;P.R.China |
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| Abstract: | The interaction between kaempferol and calf thymus DNA（ct-DNA） was studied by UV-Visible absorption and fluorescence spectroscopies. With the increasing amount of ct-DNA added to kaempferol,obvious red shift and hypochromic effect were observed in the UV-Visible absorption spectra of kaempferol. The binding constant between kaempferol and ct-DNA was obtained as 1.2×10^-4 by double-reciprocal analysis. Compared to the weak fluorescence of kaempferol, the fluorescence intensity was evidently enhanced with the combination to ct-DNA, indicating that kaempferol could be used as a fluorescence probe to investigate the interactions with biomacromolecules. |
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| Keywords: | Kaempferol ct-DNA UV-Visible Absorption Spectroscopy Fluorescence Spectroscopy |
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