Protein patterning based on electrochemical activation of bioinactive surfaces with hydroquinone-caged biotin |
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Authors: | Kim Kyuwon Yang Haesik Jon Sangyong Kim Eunkyung Kwak Juhyoun |
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Institution: | Korea Research Institute of Standards and Science, Daejeon 305-600, Korea. |
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Abstract: | We report a protein attachment and patterning method based on a hydroquinone-caged biotin surface that generates bioactive biotin by mild electrochemical perturbation. The electrochemical activation proceeds under the buffered aqueous environment at neutral pH. It also allows site-selective generation of bioactive biotin for the immobilization of target protein by using prepatterned electrode arrays. |
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