| 离子交换层析介质Q-琼脂糖凝胶的合成和吸附性能研究 |
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| 引用本文： | 浦宇,徐伟,王芝祥.离子交换层析介质Q-琼脂糖凝胶的合成和吸附性能研究[J].离子交换与吸附,2004,20(5):470-474. |
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| 作者姓名： | 浦宇 徐伟 王芝祥 |
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| 作者单位： | 上海医药工业研究院亚东核级树脂有限公司,上海,200040 |
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| 基金项目： | 国家海洋领域 863 项目 (819-07-06) |
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| 摘 要： | 以琼脂糖凝胶为基质，经2，3-环氧丙基三甲基氯化铵反应后，制得强碱型阴离子交换树脂Q-琼脂糖凝胶．考察了该反应的主要影响因素，并研究了对BSA蛋白质的吸附和对GL-7ACA酰化酶的分离性能，结果表明，其吸附性能、分离纯化性能均与国外同类产品相近。
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| 关 键 词： | 离子交换层析介质 吸附性能 Q-琼脂糖凝胶 |
| 文章编号： | 1001-5493(2004)05-0470-05 |
| 修稿时间： | 2003年11月23 |
SYSTHESIS AND ADSORPTION PROPERTIES OF Q-AGAROSE GEL |
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| PU Yu XU Wei WANG Zhixiang.SYSTHESIS AND ADSORPTION PROPERTIES OF Q-AGAROSE GEL[J].Ion Exchange and Adsorption,2004,20(5):470-474. |
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| Authors: | PU Yu XU Wei WANG Zhixiang |
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| Institution: | PU Yu XU Wei WANG Zhixiang Shanghai Institute of Pharmaceutical Industry,YaDong Nuclear Grade Resins Co. Ltd.,Shanghai 200040,China |
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| Abstract: | The Q-agarose gel was made by reacting glycidyl trimethy ammonium chloride with Sepharose 6 Fast Flow. Its main effects on the reaction were determined and the adsorption of BSA as well as the purification of GL-7ACA acylase was studied. The results showed that the adsorption capacity and the separation capability of the Q-agarose gel made by authors were similar to the imported analogous product. |
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| Keywords: | Ion exchange resin adsorption Q-agarose gel |
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