NADH screen-printed electrodes modified with zirconium phosphate,Meldola blue,and Reinecke salt. Application to the detection of glycerol by FIA |
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Authors: | A. Radoi D. Compagnone M. Batič J. Klinčar Lo Gorton G. Palleschi |
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Affiliation: | (1) Dipartimento di Scienze e Tecnologie Chimiche, Università “Tor Vergata”, via della Ricerca Scientifica 1, 00133 Rome, Italy;(2) Dipartimento di Scienze degli Alimenti, Università di Teramo, via C. Lerici 1, Mosciano S. Angelo, 64023 Teramo, Italy;(3) Food Science and Technology Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia;(4) Research Institute for Viticulture and Enology of the Ministry of Agriculture and Rural Development, Urihegy 5/A, 6001 Kecskemét, Hungary;(5) Department of Analytical Chemistry, Lund University, P.O. Box 124, 221 00 Lund, Sweden |
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Abstract: | Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10−4 mol L−1 NADH, %RSD = 4.2, n = 90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag|AgCl), flow rate (0.30 mL min−1) and pH-dependence (range 4.0–10.0) were evaluated and optimized. A glycerol biosensor, prepared by immobilizing glycerol dehydrogenase (GDH) on the working electrode area of a bMBZrPRs-SPE, was also assembled. The biosensor was most stable at pH 8.5 (%RSD = 5.6, n = 70, 0.25 mmol L−1 glycerol). The detection and quantification limits were 2.8 × 10−6 and 9.4 × 10−6 mol L−1, respectively, and the linear working range was between 1.0 × 10−5 and 1.0 × 10−4 mol L−1. To assess the effect of interferences, and recovery by the probe we analyzed samples taken during fermentation of chemically defined grape juice medium and compared the results with those obtained by HPLC. |
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Keywords: | Zirconium phosphate Meldola blue Reinecke salt NADH Glycerol biosensor |
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