Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing |
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| Authors: | Brunner Jens Kraemer Roland |
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| Affiliation: | Anorganisch-Chemisches Institut, Universit?t Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany. |
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| Abstract: | A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3'-end and a copper(II) complex to the 5'-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches. |
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