The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer |
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Authors: | Suzuki Yoshio Arakawa Hidetoshi Maeda Masako |
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Institution: | School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan. |
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Abstract: | Capillary electrophoresis has been applied the analyses of many clinical drugs due to its rapid, high-resolution separation. In this study, electrokinetic chromatography involving the combination of SDS and dextran sulfate, which are synthetic polymers, was examined in order to obtain high resolution. Use of 2% dextran sulfate (10,000 molecular weight), 20 mm SDS running buffer containing boric acid solution (pH 9.2) and a silica capillary (inner diameter of 75 micro m, effective length of 50 cm, 57 cm overall length) afforded separation of 10 kinds of benzodiazepines. The detection limit was 0.2 micro g/mL; additionally, reproducibilities were de fi ned as the peak height and migration time. The average peak height was 5.92% (2.46-17.61), whereas the average migration time was 0.44% (0.18-0.76; n = 5). This separations system can be applied to the analysis and measurement of other pharmaceuticals as well. |
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Keywords: | Capillary electrophoresis Dextran Sulfate SDS benzodiazepine affinity electrokinetic chromatography |
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