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Fluorometric Determination of Traces of Nitrite with Rhodamine 6G
Authors:Nianqin Jie  Zhikun Si  Jinghe Yang  Zhuang Miao  Xirong Huang  Qiang Zhang  Zhongqing Song
Affiliation:aDepartment of Chemistry, Shandong University, Jinan, China, 250100;bChemistry Teaching–Research Section, Dalian Maritime University
Abstract:A simple and highly selective fluorometric method for determining nitrite with rhodamine 6G is described. The method is based on the oxidation of rhodamine 6G in sulfuric acid medium. A linear calibration graph passing through the origin was obtained in the range 0.02–0.4 μg/ml nitrite. The detection limit is 0.001 μg/ml. The method is free from interference by Fe(III) and Cu(II), which normally interfere with other methods. The method was applied successfully to the determination of nitrite in tap water, lake water, and milk samples.
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