A novel three-dimensional system to study interactions between endothelial cells and neural cells of the developing central nervous system |
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Authors: | Richard Milner |
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Institution: | (1) The Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB21QP, UK;(2) Department of Molecular and Experimental Medicine,MEM-132, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, US |
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Abstract: | Background During angiogenesis in the developing central nervous system (CNS), endothelial cells (EC) detach from blood vessels growing
on the brain surface, and migrate into the expanding brain parenchyma. Brain angiogenesis is regulated by growth factors and
extracellular matrix (ECM) proteins secreted by cells of the developing CNS. In addition, recent evidence suggests that EC
play an important role in establishing the neural stem cell (NSC) niche. Therefore, two-way communication between EC and neural
cells is of fundamental importance in the developing CNS. To study the interactions between brain EC and neural cells of the
developing CNS, a novel three-dimensional (3-D) murine co-culture system was developed. Fluorescent-labelled brain EC were
seeded onto neurospheres; floating cellular aggregates that contain NSC/neural precursor cells (NPC) and smaller numbers of
differentiated cells. Using this system, brain EC attachment, survival and migration into neurospheres was evaluated and the
role of integrins in mediating the early adhesive events addressed. |
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Keywords: | |
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