| A Novel Substrate for in situ Synthesis of Oligonucleotides： Hydrolyzed Microporous Polyamide-6 Membrane |
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| 作者姓名： | SongLI JianXinTANG MeiJuJI PengHOU PengFengXIAO NongYueHE |
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| 作者单位： | KeyLaboratoryforMolecularandBiomolecularElectronicsofMinistryofEducation,SoutheastUniversity,Nanjing210096 |
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| 摘 要： | A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01mol/L NaOH/(H2O-CH3OH) mixture medium. The formation of amines (NH2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98%.
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| 关 键 词： | 低聚核苷酸 原位聚合 聚酰胺-6膜 水解作用 酶催化 耦合效率 |
A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane |
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| SongLI JianXinTANG MeiJuJI PengHOU PengFengXIAO NongYueHE.A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane[J].Chinese Chemical Letters,2004,15(7):837-840. |
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| Authors: | Song LI Jian Xin TANG Mei Ju JI Peng HOU Peng Feng XIAO Nong Yue HE
Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education Southeast University Nanjing |
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| Institution: | Song LI,Jian Xin TANG,Mei Ju JI,Peng HOU,Peng Feng XIAO,Nong Yue HE
Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education,Southeast University,Nanjing 210096 |
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| Abstract: | A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01mol/L NaOH/(H2O-CH3OH) mixture medium. The formation of amines (NH2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98 %. |
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| Keywords: | Catalyzed hydrolysis in situ synthesis of oligonucleotides coupling efficiency poly-amide-6 membrane |
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