(Coixan polysaccharide)-graft-polyethylenimine folate for tumor-targeted gene delivery |
| |
Authors: | Jiang Qiying Shi Peihua Li Chunyan Wang Qingqing Xu Fujian Yang Wantai Tang Guping |
| |
Institution: | Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China. |
| |
Abstract: | CP-PEI-FA was prepared as an effective vector for in vitro and in vivo tumor-targeted gene delivery. The structures of the polymers were characterized, and their DNA condensation capability, particle sizes, zeta potentials, cytotoxicity and in vitro/in vivo transfection were examined. The cytotoxicity of CP-PEI-FA was significantly lower than that of PEI 25 kDa and close to that of PEI 1200. The in vitro transfection of CP-PEI-FA was tested in C6 and HeLa cells (FR-positive cells) and A549 cells (FR-negative cells). CP-PEI-FA showed a high targeting specificity and good gene transfection efficiency in FR-positive cells. These results indicate that CP-PEI-FA is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA. |
| |
Keywords: | biological application of polymers graft copolymers polysaccharides |
本文献已被 PubMed 等数据库收录! |
|