Modeling the binding of peptides on carbon nanotubes and their use as protein and DNA carriers |
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Authors: | Vanesa Sanz Helen M Coley S Ravi P Silva Johnjoe McFadden |
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Institution: | (1) Faculty of Health and Medical Sciences, University of Surrey Guildford, Surrey, GU2 7XH, UK;(2) Nano-Electronics Centre, Advanced Technology Institute, University of Surrey Guildford, Surrey, GU2 7XH, UK |
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Abstract: | An in-depth study of a novel functionalization of carbon nanotubes for their application as protein and DNA carriers is presented.
First, the optimum conditions for the dispersion of single-walled carbon nanotubes (SWCNTs) with amphiphilic polypeptides
were obtained, and the SWCNT–polypeptide complexes were characterized by different techniques (UV–Vis-NIR, CD, and AFM). Based
on the properties of the SWCNT–polypeptide complexes, a model that characterizes the adsorption of natural proteins onto SWCNT
was described for the first time. This model predicts the adsorption of natural proteins on SWCNTs based on the protein structure
and composition, and therefore, allows the design of methods for the preparation of SWCNT–protein complexes. Besides, the
use of cationic-designed amphiphilic polypeptides to disperse SWCNTs is applied for subsequent and efficient binding of DNA
to carbon nanotubes by a bilayer approach. Therefore, in this article, we develop procedures for the use of SWCNTs as protein
and DNA carriers. The systems were delivered into cells showing that the efficiency of delivery is affected by the charge
of the complexes, which has important implications in the use of SWCNT as platforms for protein and DNA binding and subsequent
use as delivery systems. |
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