A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria |
| |
Authors: | Knauer Maria Ivleva Natalia P Niessner Reinhard Haisch Christoph |
| |
Institution: | (1) Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universit?t M?nchen, Marchioninistrasse 17, 81377 Munich, Germany; |
| |
Abstract: | We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria.
The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms
in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is
placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added
and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow
cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 103 to 4.3 × 105 cells/mL. The method has potential for routine application, e.g. for drinking water control. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|