| Abstract: | Miniaturization and optimization of the solvent delivery system, mixing device, and detection system for gradient elution at few μl/min is the most important objective of instrumental development in microHPLC using packed capillary columns. Instrumental solutions and evaluation of the performence of a dedicated system for automatic gradient elution with packed capillary columns are reported. Retention time precision shown buy the system results in an RSD of 0.20–0.52% for a PAH model mixture eluted under gradient conditions at few μl/min. Compositional accuracy of gradient profiles is also demonstrated. |
