Chitosan-based stationary phases in microcolumn liquid chromatography

Two kinds of cross-linked chitosan beads were evaluated as the stationary phase for microcolumn liquid chromatography. The phase with aminoalkyl substituent can be expected to function similarly to commonly known aminopropyl bonded phases. Separation of PAH with the chitosan phase bearing phenyl group substituents has been accomplished in the gradientelution mode with acetonitrile-water as the mobile phase. Planarity recognition capability comparable with that of monomeric octadecylsilica phase was observed.