Effect of collision gas pressure and collision energy on reactions between oxygen and the negative molecular ion of tetrachlorodibenzo-p-dioxins in the collision cell of a triple -quadrupole mass spectrometer

Low-energy reactive collisions between the negative molecular ion of a tetrachlorodibenzo-p-dioxin (TCDD) and oxygen inside the collision cell of a triple-stage quadrupole mass spectrometer produce a substitution ion M ? Cl + O]^?, a phenoxide ion C₆H_4-nO₂Cl_n]^?·, M ? HCl]^?·, and Cl^? by which 1,2,3,4-, 1,2,3,6/1,2,3,7- and 2,3,7,8-TCDD isomers can be distinguished either directly or on the basis of intensity ratios. The collision conditions have an important effect on the relative abundances. Energy- and pressure-resolved curves show that the ions formed by a collisionally activated reaction (CAR) process, i.e. M ? Cl + O]^? and C₆H_4-n,O₂Cl_n]^?·, are favoured by a high pressure of oxygen (3-6 mTorr) (1 Torr = 133.3 Pa) and a low collision energy (0.1-7 eV), whereas the ions formed by a collisionally activated dissociation (CAD) process, i.e. M ? HCl]^?· and Cl^?, are favoured by high pressure and high energy. By choosing a relatively low collision energy (5 eV) and high pressure (4 mTorr), the CAR and CAD ions can be clearly detected.