Polychromatic action spectrum for the induction of a mycosporine-like amino acid in a rice-field cyanobacterium, Anabaena sp |
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| Authors: | Sinha R P Sinha J P Gröniger A Häder D-P |
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| Institution: | 1. Institut für Botanik und Pharmazeutische Biologie, Friedrich-Alexander-Universität, Staudtstrasse 5, D-91058 Erlangen, Germany;2. Shriram Institute for Industrial Research, 19 University Road, Delhi-7, India;1. Walloon Agricultural Research Centre (CRA-W), Dept. of Life Sciences, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium;2. University of Liège, Gembloux Agro-Bio Tech, Walloon Center for Industrial Biology (CWBI), Passage des Déportés 2, B-5030 Gembloux, Belgium;1. Unidade de Biotecnologia e Recursos Genéticos, Instituto Nacional de Investigação Agrária e Veterinária, I.P. (INIAV), Quinta do Marquês, Av. República, 2784-505 Oeiras, Portugal;2. GeoBioTec, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal;3. Plant Stress & Biodiversity Group, LEAF—Linking Landscape, Environment, Agriculture and Food, School of Agriculture, University of Lisbon, Quinta do Marquês, Av. República, 2784-505 Oeiras, Portugal;4. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, Quinta do Marquês, 2780-157 Oeiras, Portugal;5. Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden;1. Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education, Shenyang 110016, PR China;2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China;3. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China;4. College of Chemical Engineering, Shenyang Normal University, Shenyang 110034, PR China;1. INRA, VetAgro Sup, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France;2. INRA, UR143 URZ, 97170 Petit-Bourg, Guadeloupe, France;1. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China);2. University of Chinese Academy of Sciences, Beijing 100049 (China);1. Kyushu Research Center, Forestry and Forest Products Research Institute (FFPRI), 4-11-16 Kurokami, Chuo-ku, Kumamoto 860-0862, Japan;2. Department of Forest Soils, FFPRI, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan;3. Fukushima Prefectural Forestry Research Center, 1 Nishi-jimasaka, Narita, Asaka, Koriyama, Fukushima 963-0112, Japan;4. Shikoku Research Center, FFPRI, 2-915 Asakura-Nishimachi, Kochi 780-8077, Japan |
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| Abstract: | A polychromatic action spectrum for the induction of an ultraviolet-absorbing/screening mycosporine-like amino acid (MAA) has been determined in a filamentous and heterocystous nitrogen-fixing rice-field cyanobacterium, Anabaena sp. High-performance liquid chromatographic (HPLC) studies revealed the presence of only one type of MAA, which was identified as shinorine, a bisubstituted MAA containing both glycine and serine groups having a retention time at 2.8 min and an absorption maximum at 334 nm. Exposure of cultures to simulated solar radiation in combination with various cut-off filters (WG 280, 295, 305, 320, 335, 345, GG 400, 420, 455, 475, OG 515, 530, 570, RG 645, 665 and a broad-band filter, UG 11) clearly revealed that the induction of the MAA takes place only in the UV range. Photosynthetic active radiation (PAR) had no significant impact on MAA induction. The ratio of the absorption at 334 nm (shinorine) to 665 nm (chlorophyll a) and the action spectrum also showed the induction of MAA to be UV dependent peaking in the UV-B range at around 290 nm. The results indicate that the studied cyanobacterium, Anabaena sp. may protect itself from deleterious short wavelength solar radiation by its ability to synthesize a mycosporine-like amino acid in response to UV-B radiation and thereby screen the negative effects of UV-B. |
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