普适分光光度新方法研究

本文采用紫外分光光度法与计算机联用，测试了吸光度差与浓度的对应关系，旨在证明样品放在参比池，标样放在样品室的方法可以取代标准曲线法。并且 减小误差，最主要是定量测量用标准曲线法无法进行定量测定的高浓度试样，并且不考虑△Ａ－ｃ是否满足线性关系。

STUDY ON A NEW METHOD FOR SPECTROPHOTOMETRY

In this paper, the relation between absorbance difference and concentration was measured by the ultraviolet spectrophotometer and computer made P-E company of U.S.A. The basic goal is to prove that the standard absorption curve method can be replaced by the new method, in which the sample was put in standard room and the standard material in sample room. Utilizing this method can minimize the error, most importantly, can measure the higher concentration sample, which can not be measured by the standard curve method. The new method does not require a Linear deltaA-c linear relationship.