Determination of pheophytinatonickel(II) by reversed-phase highperformance liquid chromatography |
| |
Authors: | K. Furuya N. Ohki H. Inoue T. Shirai |
| |
Affiliation: | (1) Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223 Hiyoshi, Yokohama, Japan |
| |
Abstract: | Summary HPLC determination of pheophytinatonicke(II) (Pheo-Ni) prepared by the replacement of magnesium(II) in chlorophyll with nicke(II) is described. The good separation of PheoNi was obtained by using chemically bonded C18 as the stationary phase and acetone-methanol (50∶50, vol/vol) as the mobile phase. Conventional spectrophotometric method was also used for the determination of PheoNi. For the synthetic samples prepared by mixing (pheophytinato a) nicke(II) [(Pheo-a) Ni] and (pheophytinato b) nicke(II) [(Pheo-b) Ni], analytical values obtained by the spectrophotometric method were very high compared to those obtained by HPLC. In the proposed HPLC method, (Pheo-a) Ni and (Pheo-b). Ni could be determined in the concentration range of 0.028–30μg/ml and 0.038–30μg/ml with relative standard deviations (n=10) of 3.1% and 0.8%, respectively. |
| |
Keywords: | Column liquid chromatography Pheophytinatonicke(II) Chlorophyll Pheophytin |
本文献已被 SpringerLink 等数据库收录! |
|