| 反相离子对高效液相色谱法测定血浆中的溴新斯的明 |
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| 引用本文： | 熊华蓉,谭群友,李艺,胡霓霓,张景勍. 反相离子对高效液相色谱法测定血浆中的溴新斯的明[J]. 光谱实验室, 2011, 28(3): 982-986 |
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| 作者姓名： | 熊华蓉 谭群友 李艺 胡霓霓 张景勍 |
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| 作者单位： | 1. 重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号,400016
2. 第三军医大学大坪医院野战外科研究所胸外科中心,重庆市渝中区长江支路10号,400042 |
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| 基金项目： | 教育部博士点基金资助项目(20095503120008); 重庆市自然科学基金(CSTC2007BB5092); 第三军医大学临床科研和科研创新基金(2007XG062,2007XG60) |
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| 摘 要： | 建立了血浆中溴新斯的明的反相离子对高效液相色谱测定方法。血浆样品加沉淀剂苦味酸氢氧化钠溶液,旋涡离心,经四丁基氯化铵萃取后取上清液进样测定。色谱柱为lichrospher C18（4.6mm×250mm,5μm）;流动相为庚烷磺酸钠溶液（0.01mol/L,磷酸二氢钠0.013mol/L,用磷酸调节pH 3.0）∶乙腈=77∶23,V/V;紫外检测波长260nm;流速1.0mL/min;柱温为30℃。血浆中溴新斯的明的检出限为0.005mg/L,定量下限为0.01mg/L。血浆中溴新斯的明的线性范围为0.05—10.0mg/L,r=0.9991。本方法准确、专属性强,适用于血浆中溴新斯的明的浓度测定。
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| 关 键 词： | 反相离子对高效液相色谱法 溴新斯的明 血浆样品 |
Determination of Neostigmine Bromid in Plasma by Reversed Phase Ion-Pair High Performance Liquid Chromatography |
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| XIONG Hua-Rong TAN Qun-Youa LI Yi HU Ni-Ni ZHANG Jing-Qinga. Determination of Neostigmine Bromid in Plasma by Reversed Phase Ion-Pair High Performance Liquid Chromatography[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(3): 982-986 |
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| Authors: | XIONG Hua-Rong TAN Qun-Youa LI Yi HU Ni-Ni ZHANG Jing-Qinga |
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| Affiliation: | XIONG Hua-Rong TAN Qun-Youa LI Yi HU Ni-Ni ZHANG Jing-Qing(Medicine Engineering Research Center,Key Laboratory of Biochemical & Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,P.R.China)a(Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,P.R.China) |
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| Abstract: | Neostigmine bromide in plasma was precipitated with picric acid sodium hydroxide solution before vortex,and was extracted with tetrabutylammonium chloride.After centrifuging,the supernatant was determined by reversed phase ion-pair HPLC with a lichrospher C18 column（4.6mm×250mm,5μm）,and 77% 0.01mol/L heptane-1-sulfonic acid sodium salt solution（pH was adjusted to 3.0） and 23% acetonitrile as mobile phase.The detection wavelength was 260nm,flow rate was 1.0mL/min and the column tempreture was 30℃.The detection limit of neostigmine bromide was 0.005mg/mL,the quantitation limit of neostigmine bromide in plasma was 0.01mg/L.The linear range of the neostigmine bromide plasma was 0.05—10.0mg/L,with r=0.9991.The method is rapid,accurate for determination of neostigmine bromide in plasma. |
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| Keywords: | Reversed Phase Ion-Pair High Performance Liquid Chromatography Neostigmine Bromide Plasma Sample |
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