反相高效液相色谱荧光检测法测定新型咀嚼胶片剂中右美沙芬的血浆浓度

建立反相高效液相色谱荧光检测法测定口服新型咀嚼胶片剂后血浆中右美沙芬浓度的方法。采用Hanbon C18（4.6mm×250mm,5μm）色谱柱,以乙腈-水-磷酸-三乙胺（35∶65∶0.14∶0.10,V/V/V/V）为流动相,在流速0.6mL.min-1,进样量50μL,柱温30℃,荧光激发波长（Ex）和发射波长（Em）分别为280nm和320nm条件下,测定咀嚼胶片剂中右美沙芬的兔血药浓度。药物与杂质分离效果良好、线性范围为1—1000ng.mL-1,相关系数为0.9996。方法回收率和提取回收率分别为110.0%和83.9%。当S/N=3时,右美沙芬最低检出浓度可达1ng.mL-1。本方法准确、灵敏,可满足血药浓度检测和研究药代动力学行为的需要。

Determination of Serous Dextromethorphan from New Chewing Gum Tablet by RP-HPLC with Fluorescence Detection

An method for the determination of dextromethorphan（DH） concentration after taking new chewing gum tablet in plasma was developed by reversed phase high performance liquid chromatography.Hanbon C18（4.6mm×250mm,5μm） chromatographic column was used with acetonitrile-water-phosphatic acid-triethylamine（35∶65∶0.14∶0.10,V/V/V/V） as the mobile phase at a flow rate of 0.6mL·min1 and injection volumn of 50μL.The concentration of rabbit plasma dextromethorphan from chewing gum tablet was detected under fluorescence exciting wavelength（Ex） and emission wavelength（Em） at 280nm and 320nm,respectively.The column temperature was 30℃.The drug separated well with impurities,and the linear range of DH was 1—1000ng·mL-1 with correlation coefficient of 0.9996.The lower limit of quantification（S/N=3） was 1ng·mL-1.The method recovery and extraction recovery were 109.9%,83.7%,respectively.The method is accurate,sensitive,and meets the need for detection DH concentration in rabbet plasma and for estimation of pharmacokinetic parameters.