Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine |
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Authors: | Ming-Zhou Zhang Min-Zi Wang Zong-Lun Chen Jie-Hong Fang Mei-Ming Fang Jun Liu Xiao-Ping Yu |
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Institution: | (1) Institute of Bio-safety and Food Science, College of Life Science, China Jiliang University, Hangzhou, 310018, People’s Republic of China;(2) Hangzhou DNA Sci-tech Co., Ltd., Hangzhou, 310023, People’s Republic of China;(3) Xiasha Higher Education Zone, Xueyuan Street, Hangzhou, 310018, People’s Republic of China |
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Abstract: | A multianalyte lateral-flow immunochromatographic technique using colloidal gold-labeled polyclonal antibodies was developed
for the rapid simultaneous detection of clenbuterol and ractopamine. The assay procedure could be accomplished within 5 min,
and the results of this qualitative one-step assay were evaluated visually according to whether test lines appeared or not.
When applied to the swine urines, the detection limit and the half maximal inhibitory concentration (IC50) of the test strip under an optical density scanner were calculated to be 0.1 ± 0.01 ng mL−1 and 0.1 ± 0.01 ng mL−1, 0.56 ± 0.08 ng mL−1, and 0.71 ± 0.06 ng mL−1, respectively, the cut-off levels with the naked eye of 1 ng mL−1 and 1 ng mL−1 for clenbuterol and ractopamine were observed. Parallel analysis of swine urine samples with clenbuterol and ractopamine
showed comparable results obtained from the multianalyte lateral-flow test strip and GC-MS. Therefore, the described multianalyte
lateral-flow test strip can be used as a reliable, rapid, and cost-effective on-site screening technique for the simultaneous
determination of clenbuterol and ractopamine residues in swine urine.
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