| 分子印迹微凝胶模拟酶的研究 |
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| 引用本文： | 王红飞,唐春燕,杨浩,张黎明.分子印迹微凝胶模拟酶的研究[J].高等学校化学学报,2010,31(12):2488-2493. |
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| 作者姓名： | 王红飞 唐春燕 杨浩 张黎明 |
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| 作者单位： | 1. 中山大学化学与化学工程学院, 新型聚合物材料设计合成与应用广东省高校重点实验室, 广州 510275
2. 中山大学聚合物复合材料及功能材料教育部重点实验室, 广州 510275 |
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| 基金项目： | 国家自然科学基金(批准号:20874116,20676155); 教育部高等学校博士点基金(批准号:20090171110023)资助 |
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| 摘 要： | 采用油包水反相乳液法, 以马来酸酐酯化葡聚糖-氨基吡啶偶联物(Dex-MA-AP)为功能大单体、过渡态类似物p-硝基苯磷酸酯(NPP)为模板分子、Co2+为中心离子, 制得分子印迹微凝胶模拟酶(MIGs). 用紫外光谱研究了Dex-MA-AP上吡啶功能基团与NPP之间的相互作用. 用SEM观察了MIGs的形貌和大小. 研究发现, MIGs催化活性受模板分子和交联剂用量的影响，MIGs催化p-硝基乙酸苯酯(NPA)水解反应行为可用Michaelis-Menten方程进行描述, 其最大催化水解反应速率(Vm)和Michaelis-Menten常数(Km)分别为25.1 nmol/h和0.030 mmol/L, 且具有较好的催化选择性.
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| 关 键 词： | 模拟酶 分子印迹 微凝胶 催化活性 催化选择性 |
| 收稿时间： | 2010-02-23 |
Study on Enzyme Mimics of Molecularly Imprinted Microgels |
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| WANG Hong-Fei,TANG Chun-Yan,YANG Hao,ZHANG Li-Ming.Study on Enzyme Mimics of Molecularly Imprinted Microgels[J].Chemical Research In Chinese Universities,2010,31(12):2488-2493. |
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| Authors: | WANG Hong-Fei TANG Chun-Yan YANG Hao ZHANG Li-Ming |
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| Institution: | 1. Key Laboratory of Designed Synthesis and Application of Polymer Materials, Sun Yat\|Sen University, Guangzhou 510275, China;
2. Key Laboratory for Polymeric Composite and Functional Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat\|Sen University, Guangzhou 510275, China |
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| Abstract: | The molecularly imprinted microgels(MIGs) exhibiting enzyme-like activity to the hydrolysis of p-nitrophenyl acetate(NPA) were prepared by a reverse emulsion method with maleic anhydride esterified dextran-aminopyridine conjugate(Dex-MA-AP) as the functional monomer,p-nitrophenyl phosphate(NPP) as the template and Co2+ as the coordination center.The structure of Dex-MA-AP was characterized by 1H NMR analysis.The interaction between Dex-MA-AP and NPP was investigated by UV adsorption spectra.The morphology a... |
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| Keywords: | Enzyme mimics Molecularly imprinting Microgel Catalytic activity Catalytic selectivity |
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